NB200-598
antibody from Novus Biologicals
Targeting: POLR2A
POLR2, POLRA, RPB1
Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [2]
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Validation data
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- Product number
- NB200-598 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-598, RRID:AB_2167465
- Product name
- Mouse Monoclonal RNA Polymerase II/POLR2A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7
- Reactivity
- Human, Mouse, Simian, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis.
Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.
Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.
PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia.
Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3.
Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2.
Plasma membrane associated transcription of cytoplasmic DNA.
Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoter.
DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro.
Eng J, Thibault G, Luoh SW, Gray JW, Chang YH, Chin K
Methods in molecular biology (Clifton, N.J.) 2020;2055:521-562
Methods in molecular biology (Clifton, N.J.) 2020;2055:521-562
Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.
Appanah R, Lones EC, Aiello U, Libri D, De Piccoli G
Cell reports 2020 Feb 18;30(7):2094-2105.e9
Cell reports 2020 Feb 18;30(7):2094-2105.e9
Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.
Lin JR, Izar B, Wang S, Yapp C, Mei S, Shah PM, Santagata S, Sorger PK
eLife 2018 Jul 11;7
eLife 2018 Jul 11;7
PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia.
Luo W, Chen I, Chen Y, Alkam D, Wang Y, Semenza GL
Oncotarget 2016 Feb 9;7(6):6379-97
Oncotarget 2016 Feb 9;7(6):6379-97
Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3.
Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T
Scientific reports 2013;3:2186
Scientific reports 2013;3:2186
Peripheral subnuclear positioning suppresses Tcrb recombination and segregates Tcrb alleles from RAG2.
Chan EA, Teng G, Corbett E, Choudhury KR, Bassing CH, Schatz DG, Krangel MS
Proceedings of the National Academy of Sciences of the United States of America 2013 Nov 26;110(48):E4628-37
Proceedings of the National Academy of Sciences of the United States of America 2013 Nov 26;110(48):E4628-37
Plasma membrane associated transcription of cytoplasmic DNA.
Cheng J, Torkamani A, Peng Y, Jones TM, Lerner RA
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 3;109(27):10827-31
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 3;109(27):10827-31
Proteasome regulation of dynamic transcription factor occupancy on the GnRH-stimulated luteinizing hormone beta-subunit promoter.
Walsh HE, Shupnik MA
Molecular endocrinology (Baltimore, Md.) 2009 Feb;23(2):237-50
Molecular endocrinology (Baltimore, Md.) 2009 Feb;23(2):237-50
DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro.
Reid J, Svejstrup JQ
The Journal of biological chemistry 2004 Jul 16;279(29):29875-8
The Journal of biological chemistry 2004 Jul 16;279(29):29875-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - Analysis of MCF7 cell lysata (A) using RNA polymerase II antibody at 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - Whole cell protein from HeLa (lane 1), MCF7 (lane 2), NIH-3T3 (lane 3) and Cos7 (lane 4) were separated by SDS-PAGE on a 6% gel, transferred to PVDF and probed with 2 ug/mL NB200-598. RNA Pol II reactivity (arrowhead) is observed in HeLa, MCF and 3T3 cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - An intracellular stain was performed on Daudi cells with RNA Polymerase II/POLR2A (4H8) antibody NB200-598 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RNA Polymerase II/POLR2A Antibody (4H8) [NB200-598] - An intracellular stain was performed on HeLa cells with RNA Polymerase II/POLR2A (4H8) antibody NB200-598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to phycoerythrin.