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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-301-F55 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-F55, RRID:AB_2611168
- Product name
- Anti-Hsp25/Hsp27 (MOUSE) Monoclonal Antibody - 200-301-F55
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of mouse anti-Hsp25/Hsp27 antibody. Lane 1: molecular weight markers. Lane 2: rat lung tissue lysates. Load: 35 µg per lane. Primary antibody: Hsp25/Hsp27 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 20.2 kDa/ ~24kDa for Hsp25/Hsp27. Other band(s): none.
- Validation comment
- Western Blot
