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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [1]
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- Product number
- NB100-115 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-115, RRID:AB_2166720
- Product name
- Mouse Monoclonal DNA Polymerase epsilon catalytic subunit A Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified. This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
- Reactivity
- Human, Mouse, Drosophila, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Downregulation of HIF complex in the hypothalamus exacerbates diet-induced obesity.
Stimulation of human DNA polymerase epsilon by MDM2.
Stimulation of human DNA polymerase epsilon by MDM2.
MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon.
MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon.
Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis.
Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis.
Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon.
Incorporation and excision of 9-(2-phosphonylmethoxyethyl)guanine (PMEG) by DNA polymerase delta and epsilon in vitro.
Incorporation and excision of 9-(2-phosphonylmethoxyethyl)guanine (PMEG) by DNA polymerase delta and epsilon in vitro.
DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.
DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.
Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon.
Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon.
Further characterization of HeLa DNA polymerase epsilon.
Further characterization of HeLa DNA polymerase epsilon.
Gaspar JM, Mendes NF, Corrêa-da-Silva F, Lima-Junior JC, Gaspar RC, Ropelle ER, Araujo EP, Carvalho HM, Velloso LA
Brain, behavior, and immunity 2018 Oct;73:550-561
Brain, behavior, and immunity 2018 Oct;73:550-561
Stimulation of human DNA polymerase epsilon by MDM2.
Asahara H, Li Y, Fuss J, Haines DS, Vlatkovic N, Boyd MT, Linn S
Nucleic acids research 2003 May 1;31(9):2451-9
Nucleic acids research 2003 May 1;31(9):2451-9
Stimulation of human DNA polymerase epsilon by MDM2.
Asahara H, Li Y, Fuss J, Haines DS, Vlatkovic N, Boyd MT, Linn S
Nucleic acids research 2003 May 1;31(9):2451-9
Nucleic acids research 2003 May 1;31(9):2451-9
MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon.
Vlatkovic N, Guerrera S, Li Y, Linn S, Haines DS, Boyd MT
Nucleic acids research 2000 Sep 15;28(18):3581-6
Nucleic acids research 2000 Sep 15;28(18):3581-6
MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon.
Vlatkovic N, Guerrera S, Li Y, Linn S, Haines DS, Boyd MT
Nucleic acids research 2000 Sep 15;28(18):3581-6
Nucleic acids research 2000 Sep 15;28(18):3581-6
Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis.
Liu W, Linn S
Nucleic acids research 2000 Nov 1;28(21):4180-8
Nucleic acids research 2000 Nov 1;28(21):4180-8
Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis.
Liu W, Linn S
Nucleic acids research 2000 Nov 1;28(21):4180-8
Nucleic acids research 2000 Nov 1;28(21):4180-8
Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon.
Li Y, Pursell ZF, Linn S
The Journal of biological chemistry 2000 Jul 28;275(30):23247-52
The Journal of biological chemistry 2000 Jul 28;275(30):23247-52
Incorporation and excision of 9-(2-phosphonylmethoxyethyl)guanine (PMEG) by DNA polymerase delta and epsilon in vitro.
Kramata P, Downey KM, Paborsky LR
The Journal of biological chemistry 1998 Aug 21;273(34):21966-71
The Journal of biological chemistry 1998 Aug 21;273(34):21966-71
Incorporation and excision of 9-(2-phosphonylmethoxyethyl)guanine (PMEG) by DNA polymerase delta and epsilon in vitro.
Kramata P, Downey KM, Paborsky LR
The Journal of biological chemistry 1998 Aug 21;273(34):21966-71
The Journal of biological chemistry 1998 Aug 21;273(34):21966-71
DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.
Islas L, Fairley CF, Morgan WF
Nucleic acids research 1998 Aug 15;26(16):3729-38
Nucleic acids research 1998 Aug 15;26(16):3729-38
DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination.
Islas L, Fairley CF, Morgan WF
Nucleic acids research 1998 Aug 15;26(16):3729-38
Nucleic acids research 1998 Aug 15;26(16):3729-38
Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon.
Li Y, Asahara H, Patel VS, Zhou S, Linn S
The Journal of biological chemistry 1997 Dec 19;272(51):32337-44
The Journal of biological chemistry 1997 Dec 19;272(51):32337-44
Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon.
Li Y, Asahara H, Patel VS, Zhou S, Linn S
The Journal of biological chemistry 1997 Dec 19;272(51):32337-44
The Journal of biological chemistry 1997 Dec 19;272(51):32337-44
Further characterization of HeLa DNA polymerase epsilon.
Chui G, Linn S
The Journal of biological chemistry 1995 Apr 7;270(14):7799-808
The Journal of biological chemistry 1995 Apr 7;270(14):7799-808
Further characterization of HeLa DNA polymerase epsilon.
Chui G, Linn S
The Journal of biological chemistry 1995 Apr 7;270(14):7799-808
The Journal of biological chemistry 1995 Apr 7;270(14):7799-808
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.
