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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-78113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562
- Concentration
- 0.34 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLE in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with POLE polyclonal antibody (Product # PA5-78113) using a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLE Polyclonal Antibody detects POLE protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLE protein stained by POLE Polyclonal Antibody (Product # PA5-78113) diluted at 1:2,500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLE Polyclonal Antibody detects POLE protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. POLE Polyclonal Antibody (Product # PA5-78113) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
