AP10478c

antibody from Abgent

Targeting: EXO5 C1orf176, DEM1, FLJ21144

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP10478c

Provider

Abgent

Proper citation

Abgent Cat#AP10478c, RRID:AB_10820588

Product name

DEM1 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

DEM1 Antibody (Center) (Cat. #AP10478c) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

DEM1 Antibody (Center) (Cat. #AP10478c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DEM1 antibody detected the DEM1 protein (arrow).

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

DEM1 Antibody (Center) (Cat. #AP10478c) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50