PAB22149

antibody from Abnova Corporation

Targeting: EXO5 C1orf176, DEM1, FLJ21144

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22149

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22149, RRID:AB_10966157

Product name

DEM1 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids of human DEM1.

Reactivity

Human

Host

Rabbit

Antigen sequence

ELPGFLAPEKAAVLDTGASIHLARELELHDLVTVP
VTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFG
EVEGVLLVGVIDELHYTAKG


Isotype

IgG

Vial size

100 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with DEM1 polyclonal antibody (Cat # PAB22149) at 1:250-1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with DEM1 polyclonal antibody (Cat # PAB22149) shows strong cytoplasmic positivity with a granular pattern in neuronal cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)