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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-63771 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MTTTTTFKGV DPNSRNSSRV LRPPGGGSNF SLGFDEPTEQ PVRKNKMASN IFGTPEENQA SWAKS Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 97%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HN1 in A-549 cells transfected with control siRNA, target specific siRNA probe #1, using a HN1 Polyclonal Antibody (Product # PA5-63771). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HN1 in human cell line PC-3 using a HN1 Polyclonal Antibody (Product # PA5-63771).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HN1 in human testis tissue shows moderate nuclear positivity in cells in seminiferus ducts. Samples were probed using a HN1 Polyclonal Antibody (Product # PA5-63771).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HN1 in human cell line A549 shows positivity in nucleus & nuclear membrane. Samples were probed using a HN1 Polyclonal Antibody (Product # PA5-63771).
