Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-32510 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32510, RRID:AB_2248204
- Product name
- Rabbit Polyclonal HN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HN1 Antibody [NBP1-32510] - (30 ug whole cell lysate) A: A431 B: H1299 C: HeLa S3 D: Hep G2 12% SDS PAGE; antibody diluted at 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HN1 Antibody [NBP1-32510] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HN1 Antibody [NBP1-32510] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HN1 Antibody [NBP1-32510] - Wild-type (WT) and HN1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HN1 Antibody [NBP1-32510] - An ELISA plate is coated with 50 uL of HN1 recombinant protein at concentration ranged from 0.004 ug/mL to 1 ug/mL. The coated protein is detected with HN1 antibody at 0.25 ug/mL.
