CABT-BL2149

LIN28B antibody from Creative Diagnostics
CSDD2, FLJ16517
Western blot
Recommended by provider
ELISA
Recommended by provider
Immunohistochemistry
Recommended by provider
Flow cytometry
Recommended by provider

Antibody data

Product number
CABT-BL2149
Provider
Creative Diagnostics
Product name
Anti-LIN28B (internal region) polyclonal antibody
Provider product page
Creative Diagnostics - CABT-BL2149
Antibody type
Polyclonal
Description
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5-GGAG-3 motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5-GGAG-3 motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Reactivity
Human
Host
Rabbit
Conjugate
Unconjugated
Isotype
IgG
Storage
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.