PA5-81548

antibody from Invitrogen Antibodies

Targeting: SUMO2 SMT3B, SMT3H2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-81548

Provider

Invitrogen Antibodies

Product name

SUMO2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SUMO2 in Lane A: SW480 Whole Cell Lysate (30 µg). Samples were probed using a SUMO2 Polyclonal Antibody (Product # PA5-81548) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:12 kDa. Observed band size:17 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SUMO2 Polyclonal Antibody (Product # PA5-81548) at 1:500 dilution. Lane A: SW480 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 12 kDa. Observed band size: 17 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of SUMO2 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with SUMO2 Polyclonal Antibody (Product # PA5-81548, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SUMO2 Immunoprecipitation using: Lane A: 0.5 mg SW480 Whole Cell Lysate 2 µL with SUMO2 Polyclonal Antibody (Product # PA5-81548) and 15 µL of 50 % Protein G agarose. Primary antibody: SUMO2 Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa.