BML-PW9465-0025

antibody from Enzo Life Sciences

Targeting: SUMO2 SMT3B, SMT3H2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BML-PW9465-0025

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#BML-PW9465-0025, RRID:AB_2052614

Product name

SUMO-2/3 (human) (NT) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Partially purified by salt precipitation.

Reactivity

Human

Host

Rabbit

Vial size

25 μl

Storage

-20°C

References

Submitted references

ATPase-dependent control of the Mms21 SUMO ligase during DNA repair.

Bermúdez-López M, Pociño-Merino I, Sánchez H, Bueno A, Guasch C, Almedawar S, Bru-Virgili S, Garí E, Wyman C, Reverter D, Colomina N, Torres-Rosell J
PLoS biology 2015 Mar;13(3):e1002089

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western Blot Analysis: SUMOylation assay utilising His6-tagged SUMO-2 (BML-UW9205), SUMO E1 (BML-UW9330), SUMO E2 (BML-UW9320) and RANGAP1 as substrate in presence (lane 2) and absence (lanes 1) of ATP after SDS-PAGE and blotting to PVDF with subsequent probing with BML-PW9465.