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LearnMAB5856
antibody from R&D Systems
Targeting: PLXNA4
DKFZp434G0625PRO34003, FAYV2820, KIAA1550, PLXNA4A, PLXNA4B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB5856 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Plexin A4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Plexin A4 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) Plexin A1 is observed. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Plexin B1, B2, B3, C1, D1, rmPlexin A2, and A3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q9HCM2- Isotype
- IgG
- Antibody clone number
- 707201
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex.
Podojil JR, Chiang MY, Ifergan I, Copeland R, Liu LN, Maloveste S, Langermann S, Liebenson D, Balabanov R, Chi H, Chen L, Vignali DAA, Miller SD
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):897-907
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):897-907
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Plexin A4 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Plexin A4 Monoclonal Antibody (Catalog # MAB5856) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin A4 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
