Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6325 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6325, RRID:AB_1573966
- Product name
- CHFR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of CHFR.
- Antigen sequence
KFNHICEQTRFKN
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
Chfr defines a mitotic stress checkpoint that delays entry into metaphase.
Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T
The Journal of biological chemistry 2012 Apr 13;287(16):12975-84
The Journal of biological chemistry 2012 Apr 13;287(16):12975-84
Chfr defines a mitotic stress checkpoint that delays entry into metaphase.
Scolnick DM, Halazonetis TD
Nature 2000 Jul 27;406(6794):430-5
Nature 2000 Jul 27;406(6794):430-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CHFR polyclonal antibody (Cat # PAB6325) staining (2.5 ug/mL) of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)