H00055743-M01

antibody from Novus Biologicals

Targeting: CHFR FLJ10796, RNF196

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00055743-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00055743-M01, RRID:AB_2080812

Product name

Mouse Monoclonal CHFR Antibody

Antibody type

Monoclonal

Description

IgG purified. CHFR - checkpoint with forkhead and ring finger domains

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells.

Woo SR, Lee HJ, Oh SJ, Kim S, Park SH, Lee J, Song KH, Kim TW
Biochemical and biophysical research communications 2018 Sep 10;503(3):1812-1818

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Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers.

Zhang D, Xu XL, Li F, Sun HC, Cui YQ, Liu S, Xu PY
Oncology letters 2017 Dec;14(6):8042-8050

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Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach.

Fujita A, Yamamoto H, Imamura M, Nakamura N, Maehara Y, Tsuneyoshi M, Oda Y
Virchows Archiv : an international journal of pathology 2012 Feb;460(2):163-9

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CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.

Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T
The Journal of biological chemistry 2012 Apr 13;287(16):12975-84

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Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer.

Hu SL, Huang DB, Sun YB, Wu L, Xu WP, Yin S, Chen J, Jiang XD, Shen G
Medical oncology (Northwood, London, England) 2011 Jun;28(2):447-54

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Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.

Privette LM, Weier JF, Nguyen HN, Yu X, Petty EM
Neoplasia (New York, N.Y.) 2008 Jul;10(7):643-52

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Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.

Privette LM, Weier JF, Nguyen HN, Yu X, Petty EM
Neoplasia (New York, N.Y.) 2008 Jul;10(7):643-52

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Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.

Gao YJ, Xin Y, Zhang JJ, Zhou J
World journal of gastroenterology 2008 Aug 28;14(32):5000-7

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Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.

Gao YJ, Xin Y, Zhang JJ, Zhou J
World journal of gastroenterology 2008 Aug 28;14(32):5000-7

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Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.

Milne AN, Sitarz R, Carvalho R, Polak MM, Ligtenberg M, Pauwels P, Offerhaus GJ, Weterman MA
Human pathology 2007 Jun;38(6):903-13

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Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression.

Privette LM, González ME, Ding L, Kleer CG, Petty EM
Cancer research 2007 Jul 1;67(13):6064-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.