- NBP1-92337 - Novus Biologicals RMND5A antibody

Submitted references The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation.

Maitland MER, Onea G, Chiasson CA, Wang X, Ma J, Moor SE, Barber KR, Lajoie GA, Shaw GS, Schild-Poulter C
Scientific reports 2019 Jul 8;9(1):9864

Regulation of c-Raf Stability through the CTLH Complex.

McTavish CJ, Bérubé-Janzen W, Wang X, Maitland MER, Salemi LM, Hess DA, Schild-Poulter C
International journal of molecular sciences 2019 Feb 21;20(4)

Inhibition of HDAC6 activity through interaction with RanBPM and its associated CTLH complex.

Salemi LM, Maitland MER, Yefet ER, Schild-Poulter C
BMC cancer 2017 Jul 1;17(1):460

RMND5 from Xenopus laevis is an E3 ubiquitin-ligase and functions in early embryonic forebrain development.

Pfirrmann T, Villavicencio-Lorini P, Subudhi AK, Menssen R, Wolf DH, Hollemann T
PloS one 2015;10(3):e0120342

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RMND5A Antibody [NBP1-92337] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RMND5A Antibody [NBP1-92337] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RMND5A Antibody [NBP1-92337] - rmnd5 is expressed during early embryonic development. Rmnd5 protein at different developmental stages. Western blot analysis of embryo lysate from indicated stages (top). alpha-RMND5A (Novus Biological; rabbit, 1:1000); alpha-Tubulin (at, 1:2500). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120342), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RMND5A Antibody [NBP1-92337] - rmnd5 is expressed during early embryonic development. Temporal RT-PCR analysis of rmnd5 expression (top panel); different developmental stages (NF-stages) indicated at the top. ODC1 functions as RNA input control (bottom). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120342), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: RMND5A Antibody [NBP1-92337] - Rmnd5 is part of an ubiquitin ligase complex. Glycerol step gradient of Xenopus laevis NF stage 36 embryo lysates. Molecular mass (MW) standard: albumin (67 kDa), fraction 1, 2; LDH (140 kDa), fraction 4; catalase (232 kDa), fraction 6,7. Western blot analysis with alpha-RMND5A (Rmnd5; upper panel) (1:1000) and alpha-ARMC8 (lower panel) (1:1000). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120342), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: RMND5A Antibody [NBP1-92337] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

NBP1-92337

Antibody data