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LearnPA5-97124
antibody from Invitrogen Antibodies
Targeting: CHCHD3
FLJ20420, Mic19, MICOS19, MINOS3, PPP1R22
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-97124 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHCHD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MGGTTSTRRV TFEADENENI TVVKGIRLSE NVIDRMKESS PSGSKSQRYS GAYGASVSDE ELKRRVAEEL ALEQAKKESE DQKRLKQAKE LDRERAAANE QLTRAILRER ICSEEERAKA KHLARQLEEK DRVLKKQDAF YKEQLARLEE RSSEFYRVTT EQYQKAAEEV EAKFKRYESH PVCADLQAKI LQCYRENTHQ TLKCSALATQ YMHCVNHAKQ SMLEKGG; Positive Samples: A-549, HeLa, A-431, Jurkat, HepG2, mouse kidney, mouse liver, rat heart, rat kidney, Mouse kidney, Mouse liver, Mouse heart; Cellular Location: Cytoplasm, Intermembrane side, Lipid-anchor, Mitochondrion, Mitochondrion inner membrane, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHCHD3 in extracts of various cell lines using CHCHD3 Polyclonal Antibody (Product # PA5-97124) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHCHD3 was performed in U2OS cells using CHCHD3 Polyclonal Antibody (Product # PA5-97124) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHCHD3 in paraffin-embedded mouse brain using CHCHD3 Polyclonal Antibody (Product # PA5-97124) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHCHD3 in paraffin-embedded rat brain using CHCHD3 Polyclonal Antibody (Product # PA5-97124) at a dilution of 1:100.
