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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX115653 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115653, RRID:AB_10619587
- Product name
- SCMH1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX115653 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX115653 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SCMH1 antibody (GTX115653) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SCMH1 antibody immunoprecipitates SCMH1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of SCMH1 protein by 2.5 ?g of SCMH1 antibody (GTX115653) 7.5% SDS-PAGE The immunoprecipitated SCMH1 protein was detected by SCMH1 antibody (GTX115653) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
