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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 28216-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TTC12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDADKEKDL QKFLKNVDEI SNLIQEMNSD DPVVQQKAVL ETEKRLLLME EDQEEDECRT TLNKTMISPP QTALKSAEEI NSEAFLASVE KDAKERAKRR RENKVLADAL KEKGNEAFAE GNYETAILRY SEGLEKLKDM KVLYTNRAQA YMKLEDYEKA LVDCEWALKC DEKCTKAYFH MGKANLALKN YSVSRECYKK ILEINPKLQT QVKGYLNQVD LQEKADLQEK EAHELLDSGK NTAVTTKNLL ETLSKPDQ (1-257 aa encoded by B C032355)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.47 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 28216-1-AP (TTC12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using (Product # 28216-1-AP) (TTC12 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
