13978-1-AP

antibody from Invitrogen Antibodies

Targeting: SEC63 DNAJC23, ERdj2, PRO2507, SEC63L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13978-1-AP

Provider

Invitrogen Antibodies

Product name

SEC63 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IQDLVSLKE SDRHTLLHFL EDEKYEEVMA VLGSFPYVTM DIKSQVLDDE DSNNITVGSL VTVLVKLTRQ TMAEVFEKEQ SICAAEEQPA EDGQGETNKN RTKGGWQQKS KGPKKTAKSK KKKPLKKKPT PVLLPQSKQQ KQKQANGVVG NEAAVKEDEE EVSDKGSDSE EEETNRDSQS EKDDGSDRDS DREQDEKQNK DDEAEWQELQ QSIQRKERAL LETKSKITHP VYSLYFPEEK QEWWWLYIAD RKEQTLISMP YHVCTLKDTE EVELKFPAPG KPGNYQYTVF LRSDSYMGLD QIKPLKLEVH EAKPVPENHP QWDTAIEGDE DQEDSEGFED SFEEEEEEEE DDD (409-760 aa encoded by BC047221)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (Product # 13978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SEC63 (IP:13978-1-AP, 4ug; Detection:13978-1-AP 1:300) with A431 cells lysate 2400ug.