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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 17251-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Collagen Type VIII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GIPGPMGPP GAIGFPGPKG EGGIVGPQGP PGPKGEPGLQ GFPGKPGFLG EVGPPGMRGL PGPIGPKGEA GQKGVPGLPG VPGLLGPKGE PGIPGDQGLQ GPPGIPGIGG PSGPIGPPGI PGPKGEPGLP GPPGFPGIGK PGVAGLHGPP GKPGALGPQG QPGLPGPPGP PGPPGPPAVM PPTPPPQGEY LPDMGLGIDG VKPPHAYGAK KGKNGGPAYE MPAFTAELTA PFPPVGAPVK FNKLLYNGRQ NYNPQTGIFT CEVPGVYYFA YHVHCKGGNV WVALFKNNEP VMYTYDEYKK GFLDQASGSA VLLLRPGDRV FLQMPSEQAA GLYAGQYVHS SFSGYLLYPM (396-744 aa encoded by BC013581)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 17251-1-AP (Collagen Type VIII antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Collagen Type VIII (IP:17251-1-AP, 4ug; Detection:17251-1-AP 1:500) with HepG2 cells lysate 1800ug.
