Ab122942

antibody from Aladdin Scientific Corporation

Targeting: AKT1S1 Lobe, MGC2865, PRAS40

Western blot (Data presented on provider website)

Antibody Data

Product number

Ab122942

Provider

Aladdin Scientific Corporation

Product name

Recombinant PRAS40 Antibody

Antibody type

Recombinant

Description

Rabbit anti Human PRAS40 Antibody, Recombinant (R05-3A5), could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 237-249


Antibody clone number

R05-3A5

Vial size

100μl,10μl,1ml,50μl

Concentration

1,4 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.