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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB6890 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Phospho-PRAS40 (T246) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse PRAS40 when phosphorylated at T246
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 760502
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells.
Targeted depletion of PIK3R2 induces regression of lung squamous cell carcinoma.
Pio GM, Xia Y, Piaseczny MM, Chu JE, Allan AL
PloS one 2017;12(5):e0177640
PloS one 2017;12(5):e0177640
Targeted depletion of PIK3R2 induces regression of lung squamous cell carcinoma.
Vallejo-Díaz J, Olazabal-Morán M, Cariaga-Martínez AE, Pajares MJ, Flores JM, Pio R, Montuenga LM, Carrera AC
Oncotarget 2016 Dec 20;7(51):85063-85078
Oncotarget 2016 Dec 20;7(51):85063-85078
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 20 minutes and 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PRAS40 (T246) Monoclonal Antibody (Catalog # MAB6890) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PRAS40 (T246) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Phospho-PRAS40 (T246) in MCF-7 Human Cell Line. PRAS40 phosphorylated at T246 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse Phospho-PRAS40 (T246) Monoclonal Antibody (Catalog # MAB6890) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
