PAB2222

antibody from Abnova Corporation

Targeting: KDM2A CXXC8, DKFZP434M1735, FBL11, FBL7, FBXL11, FLJ00115, JHDM1A, KIAA1004, LILINA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2222

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2222, RRID:AB_1574823

Product name

FBXL11 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of FBXL11.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Histone demethylation by a family of JmjC domain-containing proteins.

Tsukada Y, Fang J, Erdjument-Bromage H, Warren ME, Borchers CH, Tempst P, Zhang Y
Nature 2006 Feb 16;439(7078):811-6

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Nucleolar proteome dynamics.

Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M
Nature 2005 Jan 6;433(7021):77-83

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of FBXL11 (arrow) using rabbit FBXL11 polyclonal antibody (Cat # PAB2222). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JHDM1a/FBXL11 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human testis tissue reacted with FBXL11 polyclonal antibody (Cat # PAB2222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)