PAB23095

antibody from Abnova Corporation

Targeting: NIT2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23095

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23095, RRID:AB_11121739

Product name

NIT2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NIT2.

Antigen sequence

YLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKI
HLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRV
GLGICYDMRFAELAQIYAQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NIT2 polyclonal antibody (Cat # PAB23095) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NIT2 polyclonal antibody (Cat # PAB23095) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with NIT2 polyclonal antibody (Cat # PAB23095) shows strong cytoplasmic positivity in hepatocytes at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)