CF501097

antibody from Invitrogen Antibodies

Targeting: NIT2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF501097

Provider

Invitrogen Antibodies

Product name

NIT2 Monoclonal Antibody (OTI3D5), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3D5

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2. Positive lysates LY402761 (100 µg) and LC402761 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-NIT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). (1:200)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NIT2-Knockout 293T cells (KO, Cat# LC812235) were separated by SDS-PAGE and immunoblotted with anti-NIT2 monoclonal antibody TA501097, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-NIT2 mouse monoclonal antibody (TA501097) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501097, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501097, Dilution 1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (TA501098, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY NIT2(RC210660)(Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT2 mouse monoclonal(TA501097), and then analyzed by flow cytometry.