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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00027244-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00027244-B01P, RRID:AB_1237945
- Product name
- SESN1 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human SESN1 protein.
- Antigen sequence
MAEGENEVRWDGLCSRDSTTRETALENIRQTILRK
TEYLRSVKETPHRPSDGLSNTESSDGLNKLLAHLL
MLSKRCPFKDVREKSEFILKSIQELGIRIPRPLGQ
GPSRFIPEKEILQVGSEDAQMHALFADSFAALGRL
DNITLVMVFHPQYLESFLKTQHYLLQMDGPLPLHY
RHYIGIMAAARHQCSYLVNLHVNDFLHVGGDPKWL
NGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGL
LKAEEHSWSLAELVHAVVLLTHYHSLASFTFGCGI
SPEIHCDGGHTFRPPSVSNYCICDITNGNHSVDEM
PVNSAENVSVSDSFFEVEALMEKMRQLQECRDEEE
ASQEEMASRFEIEKRESMFVFSSDDEEVTPARAVS
RHFEDTSYGYKDFSRHGMHVPTFRVQDYCWEDHGY
SLVNRLYPDVGQLIDEKFHIAYNLTYNTMAMHKDV
DTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDR
SFKVYIKTVVCTPEKVTKRMYDSFWRQFKHSEKVH
VNLLLIEARMQAELLYALRAITRYMT- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references IGF-1 receptor antagonism inhibits autophagy.
Renna M, Bento CF, Fleming A, Menzies FM, Siddiqi FH, Ravikumar B, Puri C, Garcia-Arencibia M, Sadiq O, Corrochano S, Carter S, Brown SD, Acevedo-Arozena A, Rubinsztein DC
Human molecular genetics 2013 Nov 15;22(22):4528-44
Human molecular genetics 2013 Nov 15;22(22):4528-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SESN1 expression in transfected 293T cell line (H00027244-T01) by SESN1 MaxPab polyclonal antibody.Lane 1: SESN1 transfected lysate(60.61 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to SESN1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
