H00055684-M01

antibody from Abnova Corporation

Targeting: RABL6 bA216L13.9, C9orf86, FLJ10101, FLJ13045, Parf, pp8875, RBEL1

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00055684-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00055684-M01, RRID:AB_426016

Product name

C9orf86 monoclonal antibody (M01), clone 2E11-1F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant C9orf86.

Antigen sequence

MKIVIRGDRNTGKTALWHRLQGRPFVEEYIPTQEI
QVTSIHWSYKTTDDIVKVEVWDVVDKGKCKKRGDG
LKMENDPQEAESEMALDAEFLDVYKNCNGVVMMFD
ITKQWTFNYILRELPKVPTHVPVCVLGNYRDMGEH
RVILPDDVRDFIDNLDRPPGSSYFRYAESSMKNSF
GLKYLHKFFNIPFLQLQRETLLRQLETNQLDMDAT
LEELSVQQETEDQNYGIFLEMMEARSRGHASPLAA
NGQSPSPGSQSPVVPAGAVSTGSSSPGTPQPAPQL
PLNAAPPSSVPPVPPSEALPPPACPSAPAPRRSII
SRPPPWGWRLRGALGRRGQWPPWGGGRACHCLGRH
LPLYHRLCRCPVAAVCASELEEAGHWWSPGWALQV
LGLQAQCEPALQEGRGQLASARLGGHPGPLGAEPP
VFLRDVTEAQEGPVRVCLQRLGRGRLAVGCALPRH
LLALRAHLGPQHAYGSASGREP


Isotype

IgG

Antibody clone number

2E11-1F10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Down-regulation of c9orf86 in human breast cancer cells inhibits cell proliferation, invasion and tumor growth and correlates with survival of breast cancer patients.

Li YY, Fu S, Wang XP, Wang HY, Zeng MS, Shao JY
PloS one 2013;8(8):e71764

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged C9orf86 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to C9orf86 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol