20848-1-AP

antibody from Invitrogen Antibodies

Targeting: RABL6 bA216L13.9, C9orf86, FLJ10101, FLJ13045, Parf, pp8875, RBEL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20848-1-AP

Provider

Invitrogen Antibodies

Product name

C9orf86 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MFSALKKLV GSDQAPGRDK NIPAGLQSMN QALQRRFAKG VQYNMKIVIR GDRNTGKTAL WHRLQGRPFV EEYIPTQEIQ VTSIHWSYKT TDDIVKVEVW DVVDKGKCKK RGDGLKMEND PQEAESEMAL DAEFLDVYKN CNGVVMMFDI TKQWTFNYIL RELPKVPTHV PVCVLGNYRD MGEHRVILPD DVRDFIDNLD RPPGSSYFRY AESSMKNSFG LKYLHKFFNI PFLQLQRETL LRQLETNQLD MDATLEELSV QQETEDQNYG IFLEMMEARS RGHASPLAAN GQSPSPGSQS PVVPAGAVST (1-309 aa encoded by BC002945)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20848-1-AP ( C9orf86 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 20848-1-AP (C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 20848-1-AP (C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20848-1-AP ( C9orf86 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 20848-1-AP ( C9orf86 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).