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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- TA501842 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501842, RRID:AB_11127166
- Product name
- MICAL1 mouse monoclonal antibody, clone OTI6H4 (formerly 6H4)
- Antibody type
- Monoclonal
- Description
- MICAL1 mouse monoclonal antibody, clone OTI6H4 (formerly 6H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MICAL1
- Isotype
- IgG
- Antibody clone number
- OTI6H4
- Vial size
- 100 µl
- Concentration
- 0.93 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-MICAL1 mouse monoclonal antibody (TA501842) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1(RC208308).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501842)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501842)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501842)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC208308 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MICAL1 antibody(TA501842), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody(TA501842),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
