Antibody data
- Antibody Data
- Antigen structure
- References [36]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MAB4470 - Provider product page
- Provider
- R&D Systems
- Product name
- Myosin Heavy Chain Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- MF20
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations.
Epigenetic Regulation of the PTEN-AKT-RAC1 Axis by G9a Is Critical for Tumor Growth in Alveolar Rhabdomyosarcoma.
IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway.
Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects.
Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy.
MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In Vivo.
p300 Mediates Muscle Wasting in Lewis Lung Carcinoma.
The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts.
Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons.
Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia.
OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13.
The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice.
HSP90 modulates the myosin replacement rate in myofibrils.
Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration.
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients.
Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors.
Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation.
Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90.
NFκB signaling in alveolar rhabdomyosarcoma.
R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways.
Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation.
Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability.
Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy.
The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
Activin A induces skeletal muscle catabolism via p38β mitogen-activated protein kinase.
Mesenchymal Stem Cells From Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
Novel Therapeutic Effects of Non-thermal atmospheric pressure plasma for Muscle Regeneration and Differentiation.
CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma.
Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1.
Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma.
Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma.
Molecular ties between the cell cycle and differentiation in embryonic stem cells.
Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
Myoblasts derived from normal hESCs and dystrophic hiPSCs efficiently fuse with existing muscle fibers following transplantation.
Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle.
Myocilin interacts with syntrophins and is member of dystrophin-associated protein complex.
Dmitrieva RI, Lelyavina TA, Komarova MY, Galenko VL, Ivanova OA, Tikanova PA, Khromova NV, Golovkin AS, Bortsova MA, Sergushichev A, Sitnikova MY, Kostareva AA
Stem cells international 2019;2019:5690345
Stem cells international 2019;2019:5690345
Epigenetic Regulation of the PTEN-AKT-RAC1 Axis by G9a Is Critical for Tumor Growth in Alveolar Rhabdomyosarcoma.
Bhat AV, Palanichamy Kala M, Rao VK, Pignata L, Lim HJ, Suriyamurthy S, Chang KT, Lee VK, Guccione E, Taneja R
Cancer research 2019 May 1;79(9):2232-2243
Cancer research 2019 May 1;79(9):2232-2243
IMB0901 inhibits muscle atrophy induced by cancer cachexia through MSTN signaling pathway.
Liu D, Qiao X, Ge Z, Shang Y, Li Y, Wang W, Chen M, Si S, Chen SZ
Skeletal muscle 2019 Mar 28;9(1):8
Skeletal muscle 2019 Mar 28;9(1):8
Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects.
Ooi J, Langley SR, Xu X, Utami KH, Sim B, Huang Y, Harmston NP, Tay YL, Ziaei A, Zeng R, Low D, Aminkeng F, Sobota RM, Ginhoux F, Petretto E, Pouladi MA
Cell reports 2019 Feb 26;26(9):2494-2508.e7
Cell reports 2019 Feb 26;26(9):2494-2508.e7
Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy.
Estañ MC, Fernández-Núñez E, Zaki MS, Esteban MI, Donkervoort S, Hawkins C, Caparros-Martin JA, Saade D, Hu Y, Bolduc V, Chao KR, Nevado J, Lamuedra A, Largo R, Herrero-Beaumont G, Regadera J, Hernandez-Chico C, Tizzano EF, Martinez-Glez V, Carvajal JJ, Zong R, Nelson DL, Otaify GA, Temtamy S, Aglan M, Issa M, Bönnemann CG, Lapunzina P, Yoon G, Ruiz-Perez VL
Nature communications 2019 Feb 15;10(1):797
Nature communications 2019 Feb 15;10(1):797
MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In Vivo.
Fiedler LR, Chapman K, Xie M, Maifoshie E, Jenkins M, Golforoush PA, Bellahcene M, Noseda M, Faust D, Jarvis A, Newton G, Paiva MA, Harada M, Stuckey DJ, Song W, Habib J, Narasimhan P, Aqil R, Sanmugalingam D, Yan R, Pavanello L, Sano M, Wang SC, Sampson RD, Kanayaganam S, Taffet GE, Michael LH, Entman ML, Tan TH, Harding SE, Low CMR, Tralau-Stewart C, Perrior T, Schneider MD
Cell stem cell 2019 Apr 4;24(4):579-591.e12
Cell stem cell 2019 Apr 4;24(4):579-591.e12
p300 Mediates Muscle Wasting in Lewis Lung Carcinoma.
Sin TK, Zhu JZ, Zhang G, Li YP
Cancer research 2019 Apr 1;79(7):1331-1342
Cancer research 2019 Apr 1;79(7):1331-1342
The Major Lysosomal Membrane Proteins LAMP-1 and LAMP-2 Participate in Differentiation of C2C12 Myoblasts.
Sakane H, Akasaki K
Biological & pharmaceutical bulletin 2018;41(8):1186-1193
Biological & pharmaceutical bulletin 2018;41(8):1186-1193
Microphysiological 3D model of amyotrophic lateral sclerosis (ALS) from human iPS-derived muscle cells and optogenetic motor neurons.
Osaki T, Uzel SGM, Kamm RD
Science advances 2018 Oct;4(10):eaat5847
Science advances 2018 Oct;4(10):eaat5847
Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia.
Chen R, Xu J, She Y, Jiang T, Zhou S, Shi H, Li C
International journal of molecular medicine 2018 May;41(5):2565-2572
International journal of molecular medicine 2018 May;41(5):2565-2572
OR2H2 regulates the differentiation of human myoblast cells by its ligand aldehyde 13-13.
Kalbe B, Osterloh M, Schulz VM, Altmüller J, Becker C, Osterloh S, Hatt H
Archives of biochemistry and biophysics 2018 May 1;645:72-80
Archives of biochemistry and biophysics 2018 May 1;645:72-80
The exercise-inducible bile acid receptor Tgr5 improves skeletal muscle function in mice.
Sasaki T, Kuboyama A, Mita M, Murata S, Shimizu M, Inoue J, Mori K, Sato R
The Journal of biological chemistry 2018 Jun 29;293(26):10322-10332
The Journal of biological chemistry 2018 Jun 29;293(26):10322-10332
HSP90 modulates the myosin replacement rate in myofibrils.
Ojima K, Ichimura E, Suzuki T, Oe M, Muroya S, Nishimura T
American journal of physiology. Cell physiology 2018 Jul 1;315(1):C104-C114
American journal of physiology. Cell physiology 2018 Jul 1;315(1):C104-C114
Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration.
Koide M, Hagiwara Y, Tsuchiya M, Kanzaki M, Hatakeyama H, Tanaka Y, Minowa T, Takemura T, Ando A, Sekiguchi T, Yabe Y, Itoi E
The Tohoku journal of experimental medicine 2018 Jan;244(1):15-24
The Tohoku journal of experimental medicine 2018 Jan;244(1):15-24
Modulation of Protein Quality Control and Proteasome to Autophagy Switch in Immortalized Myoblasts from Duchenne Muscular Dystrophy Patients.
Wattin M, Gaweda L, Muller P, Baritaud M, Scholtes C, Lozano C, Gieseler K, Kretz-Remy C
International journal of molecular sciences 2018 Jan 7;19(1)
International journal of molecular sciences 2018 Jan 7;19(1)
Premyogenic progenitors derived from human pluripotent stem cells expand in floating culture and differentiate into transplantable myogenic progenitors.
Sakai-Takemura F, Narita A, Masuda S, Wakamatsu T, Watanabe N, Nishiyama T, Nogami K, Blanc M, Takeda S, Miyagoe-Suzuki Y
Scientific reports 2018 Apr 26;8(1):6555
Scientific reports 2018 Apr 26;8(1):6555
Dexamethasone Treatment at the Myoblast Stage Enhanced C2C12 Myocyte Differentiation.
Han DS, Yang WS, Kao TW
International journal of medical sciences 2017;14(5):434-443
International journal of medical sciences 2017;14(5):434-443
Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90.
Zhang G, Liu Z, Ding H, Zhou Y, Doan HA, Sin KWT, Zhu ZJ, Flores R, Wen Y, Gong X, Liu Q, Li YP
Nature communications 2017 Sep 19;8(1):589
Nature communications 2017 Sep 19;8(1):589
NFκB signaling in alveolar rhabdomyosarcoma.
Cleary MM, Mansoor A, Settelmeyer T, Ijiri Y, Ladner KJ, Svalina MN, Rubin BP, Guttridge DC, Keller C
Disease models & mechanisms 2017 Sep 1;10(9):1109-1115
Disease models & mechanisms 2017 Sep 1;10(9):1109-1115
R-spondin1 Controls Muscle Cell Fusion through Dual Regulation of Antagonistic Wnt Signaling Pathways.
Lacour F, Vezin E, Bentzinger CF, Sincennes MC, Giordani L, Ferry A, Mitchell R, Patel K, Rudnicki MA, Chaboissier MC, Chassot AA, Le Grand F
Cell reports 2017 Mar 7;18(10):2320-2330
Cell reports 2017 Mar 7;18(10):2320-2330
Functional loss of DHRS7C induces intracellular Ca2+ overload and myotube enlargement in C2C12 cells via calpain activation.
Arai S, Ikeda M, Ide T, Matsuo Y, Fujino T, Hirano K, Sunagawa K, Tsutsui H
American journal of physiology. Cell physiology 2017 Jan 1;312(1):C29-C39
American journal of physiology. Cell physiology 2017 Jan 1;312(1):C29-C39
Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability.
Ueki J, Nakamori M, Nakamura M, Nishikawa M, Yoshida Y, Tanaka A, Morizane A, Kamon M, Araki T, Takahashi MP, Watanabe A, Inagaki N, Sakurai H
Scientific reports 2017 Feb 13;7:42522
Scientific reports 2017 Feb 13;7:42522
Canadine from Corydalis turtschaninovii Stimulates Myoblast Differentiation and Protects against Myotube Atrophy.
Lee H, Lee SJ, Bae GU, Baek NI, Ryu JH
International journal of molecular sciences 2017 Dec 18;18(12)
International journal of molecular sciences 2017 Dec 18;18(12)
The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.
Kokabu S, Nakatomi C, Matsubara T, Ono Y, Addison WN, Lowery JW, Urata M, Hudnall AM, Hitomi S, Nakatomi M, Sato T, Osawa K, Yoda T, Rosen V, Jimi E
The Journal of biological chemistry 2017 Aug 4;292(31):12885-12894
The Journal of biological chemistry 2017 Aug 4;292(31):12885-12894
Activin A induces skeletal muscle catabolism via p38β mitogen-activated protein kinase.
Ding H, Zhang G, Sin KW, Liu Z, Lin RK, Li M, Li YP
Journal of cachexia, sarcopenia and muscle 2017 Apr;8(2):202-212
Journal of cachexia, sarcopenia and muscle 2017 Apr;8(2):202-212
Mesenchymal Stem Cells From Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
Boyle KE, Patinkin ZW, Shapiro AL, Baker PR 2nd, Dabelea D, Friedman JE
Diabetes 2016 Mar;65(3):647-59
Diabetes 2016 Mar;65(3):647-59
Novel Therapeutic Effects of Non-thermal atmospheric pressure plasma for Muscle Regeneration and Differentiation.
Choi JW, Kang SU, Kim YE, Park JK, Yang SS, Kim YS, Lee YS, Lee Y, Kim CH
Scientific reports 2016 Jun 28;6:28829
Scientific reports 2016 Jun 28;6:28829
CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma.
Phelps MP, Bailey JN, Vleeshouwer-Neumann T, Chen EY
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 27;113(52):15090-15095
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 27;113(52):15090-15095
Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1.
Enoki Y, Watanabe H, Arake R, Sugimoto R, Imafuku T, Tominaga Y, Ishima Y, Kotani S, Nakajima M, Tanaka M, Matsushita K, Fukagawa M, Otagiri M, Maruyama T
Scientific reports 2016 Aug 23;6:32084
Scientific reports 2016 Aug 23;6:32084
Citrus unshiu peel extract alleviates cancer-induced weight loss in mice bearing CT-26 adenocarcinoma.
Kim A, Im M, Gu MJ, Ma JY
Scientific reports 2016 Apr 11;6:24214
Scientific reports 2016 Apr 11;6:24214
Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma.
Vleeshouwer-Neumann T, Phelps M, Bammler TK, MacDonald JW, Jenkins I, Chen EY
PloS one 2015;10(12):e0144320
PloS one 2015;10(12):e0144320
Molecular ties between the cell cycle and differentiation in embryonic stem cells.
Li VC, Kirschner MW
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 1;111(26):9503-8
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 1;111(26):9503-8
Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
Yaden BC, Wang YX, Wilson JM, Culver AE, Milner A, Datta-Mannan A, Shetler P, Croy JE, Dai G, Krishnan V
The American journal of pathology 2014 Apr;184(4):1152-66
The American journal of pathology 2014 Apr;184(4):1152-66
Myoblasts derived from normal hESCs and dystrophic hiPSCs efficiently fuse with existing muscle fibers following transplantation.
Goudenege S, Lebel C, Huot NB, Dufour C, Fujii I, Gekas J, Rousseau J, Tremblay JP
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Nov;20(11):2153-67
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Nov;20(11):2153-67
Slow-dividing satellite cells retain long-term self-renewal ability in adult muscle.
Ono Y, Masuda S, Nam HS, Benezra R, Miyagoe-Suzuki Y, Takeda S
Journal of cell science 2012 Mar 1;125(Pt 5):1309-17
Journal of cell science 2012 Mar 1;125(Pt 5):1309-17
Myocilin interacts with syntrophins and is member of dystrophin-associated protein complex.
Joe MK, Kee C, Tomarev SI
The Journal of biological chemistry 2012 Apr 13;287(16):13216-27
The Journal of biological chemistry 2012 Apr 13;287(16):13216-27
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Myosin Heavy Chain in C2C12 Mouse Cell Line. Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Myosin Heavy Chain in Mouse Skeletal Muscle. Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Myosin Heavy Chain in Human Skeletal Muscle. Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.