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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00003021-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003021-M01, RRID:AB_539574
- Product name
- Mouse Monoclonal Histone H3.3B Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. H3F3B - H3 histone, family 3B (H3.3B)
- Reactivity
- Human, Mouse, Bovine, Drosophila, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Characterization of H3.3 and HIRA expression and function in bovine early embryos.
EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation.
Histone H3.3 maintains genome integrity during mammalian development.
Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis.
Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus.
Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development.
HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes.
A developmental requirement for HIRA-dependent H3.3 deposition revealed at gastrulation in Xenopus.
Cloning and characterization of glutamate receptor subunit 4 (GLUA4) and its alternatively spliced isoforms in turtle brain.
The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.
Zhang K, Wang H, Rajput SK, Folger JK, Smith GW
Molecular reproduction and development 2018 Feb;85(2):106-116
Molecular reproduction and development 2018 Feb;85(2):106-116
EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation.
Pradhan SK, Su T, Yen L, Jacquet K, Huang C, Côté J, Kurdistani SK, Carey MF
Molecular cell 2016 Jan 7;61(1):27-38
Molecular cell 2016 Jan 7;61(1):27-38
Histone H3.3 maintains genome integrity during mammalian development.
Jang CW, Shibata Y, Starmer J, Yee D, Magnuson T
Genes & development 2015 Jul 1;29(13):1377-92
Genes & development 2015 Jul 1;29(13):1377-92
Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis.
Hammoud SS, Low DH, Yi C, Carrell DT, Guccione E, Cairns BR
Cell stem cell 2014 Aug 7;15(2):239-53
Cell stem cell 2014 Aug 7;15(2):239-53
Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus.
Orsi GA, Algazeery A, Meyer RE, Capri M, Sapey-Triomphe LM, Horard B, Gruffat H, Couble P, Aït-Ahmed O, Loppin B
PLoS genetics 2013;9(2):e1003285
PLoS genetics 2013;9(2):e1003285
Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development.
Lin CJ, Conti M, Ramalho-Santos M
Development (Cambridge, England) 2013 Sep;140(17):3624-34
Development (Cambridge, England) 2013 Sep;140(17):3624-34
HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes.
Jullien J, Astrand C, Szenker E, Garrett N, Almouzni G, Gurdon JB
Epigenetics & chromatin 2012 Oct 29;5(1):17
Epigenetics & chromatin 2012 Oct 29;5(1):17
A developmental requirement for HIRA-dependent H3.3 deposition revealed at gastrulation in Xenopus.
Szenker E, Lacoste N, Almouzni G
Cell reports 2012 Jun 28;1(6):730-40
Cell reports 2012 Jun 28;1(6):730-40
Cloning and characterization of glutamate receptor subunit 4 (GLUA4) and its alternatively spliced isoforms in turtle brain.
Sabirzhanov B, Keifer J
Journal of molecular neuroscience : MN 2011 Jul;44(3):159-72
Journal of molecular neuroscience : MN 2011 Jul;44(3):159-72
The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.
Drané P, Ouararhni K, Depaux A, Shuaib M, Hamiche A
Genes & development 2010 Jun 15;24(12):1253-65
Genes & development 2010 Jun 15;24(12):1253-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.Lane 1: H3F3B transfected lysate(15 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Detection limit for recombinant GST tagged H3F3B is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H3.3B Antibody (2D7-H1) [H00003021-M01] - Analysis of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.
