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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA5-27916 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H3.3 K27M oncohistone mutant Recombinant Rabbit Monoclonal Antibody (RM192), ChIP-Verified
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Antibody clone number
- RM192
- Concentration
- 0.25 mg/mL
Submitted references A common classification framework for histone sequence alterations in tumours: an expert consensus proposal.
Leske H, Dalgleish R, Lazar AJ, Reifenberger G, Cree IA
The Journal of pathology 2021 Jun;254(2):109-120
The Journal of pathology 2021 Jun;254(2):109-120
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H3 (K27M mutant) in 293T cells transfected with WT and mutant K27M construct. Sample was incubated with Histone H3 (K27M mutant) monoclonal antibody (Product # MA5-27916) using a dilution of 0.1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H3 (K27M mutant) in 293T cells transfected with WT and mutant K27M construct. Sample was incubated with Histone H3 (K27M mutant) monoclonal antibody (Product # MA5-27916) using a dilution of 0.1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H3 (K27M mutant) in 293T cells transfected with WT and mutant K27M construct. Sample was incubated with Histone H3 (K27M mutant) monoclonal antibody (Product # MA5-27916) using a dilution of 0.1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Histone H3 (K27M mutant) in paraffin embedded 293T cells. Sample was incubated with Histone H3 (K27M mutant) monoclonal antibody (Product # MA5-27916) using a dilution of 0.01 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Histone H3 (K27M mutant) in brain tumor tissue. Sample was incubated with Histone H3 (K27M mutant) monoclonal antibody (Product # MA5-27916).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Histone H3 (K27M mutant) in brain tumor tissue. Sample was incubated with Histone H3 (K27M mutant) monoclonal antibody (Product # MA5-27916).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Antibody specificity was demonstrated by detection of enrichment of the targeted histone modification using SNAP-ChIP™ Spike-in, a proprietary technology developed by EpiCypher™. SNAP-ChIP™ spike-in was performed using H3.3 K27M Recombinant Rabbit Monoclonal Antibody (Product # MA5-27916) and H3.3 K27M was enriched compared to the other histone modifications in the SNAP-ChIP™ OncoStat Panel (Product # A47343).
