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- Antibody Data
- Antigen structure
- References [10]
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- Western blot [1]
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- Product number
- MAB2916 - Provider product page
- Provider
- R&D Systems
- Product name
- Human HTRA1/PRSS11 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human HTRA1/PRSS11 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q92743- Isotype
- IgG
- Antibody clone number
- 275615
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer's disease.
HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model.
HtrA1 Is Specifically Up-Regulated in Active Keloid Lesions and Stimulates Keloid Development.
Protective effects of an HTRA1 insertion-deletion variant against age-related macular degeneration in the Chinese populations.
High-Temperature Requirement A1 (Htra1) - A Novel Regulator of Canonical Wnt Signaling.
HTRA1 promoter variant differentiates polypoidal choroidal vasculopathy from exudative age-related macular degeneration.
Protoporphyrins enhance oligomerization and enzymatic activity of HtrA1 serine protease.
Angiographic features of transgenic mice with increased expression of human serine protease HTRA1 in retinal pigment epithelium.
Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1.
Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways.
Hondius DC, Eigenhuis KN, Morrema THJ, van der Schors RC, van Nierop P, Bugiani M, Li KW, Hoozemans JJM, Smit AB, Rozemuller AJM
Acta neuropathologica communications 2018 Jun 4;6(1):46
Acta neuropathologica communications 2018 Jun 4;6(1):46
HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model.
Melo E, Oertle P, Trepp C, Meistermann H, Burgoyne T, Sborgi L, Cabrera AC, Chen CY, Hoflack JC, Kam-Thong T, Schmucki R, Badi L, Flint N, Ghiani ZE, Delobel F, Stucki C, Gromo G, Einhaus A, Hornsperger B, Golling S, Siebourg-Polster J, Gerber F, Bohrmann B, Futter C, Dunkley T, Hiller S, Schilling O, Enzmann V, Fauser S, Plodinec M, Iacone R
EBioMedicine 2018 Jan;27:258-274
EBioMedicine 2018 Jan;27:258-274
HtrA1 Is Specifically Up-Regulated in Active Keloid Lesions and Stimulates Keloid Development.
Yamawaki S, Naitoh M, Kubota H, Aya R, Katayama Y, Ishiko T, Tamura T, Yoshikawa K, Enoshiri T, Ikeda M, Suzuki S
International journal of molecular sciences 2018 Apr 24;19(5)
International journal of molecular sciences 2018 Apr 24;19(5)
Protective effects of an HTRA1 insertion-deletion variant against age-related macular degeneration in the Chinese populations.
Ng TK, Liang XY, Lu F, Liu DT, Yam GH, Ma L, Tam PO, Chen H, Cen LP, Chen LJ, Yang Z, Pang CP
Laboratory investigation; a journal of technical methods and pathology 2017 Jan;97(1):43-52
Laboratory investigation; a journal of technical methods and pathology 2017 Jan;97(1):43-52
High-Temperature Requirement A1 (Htra1) - A Novel Regulator of Canonical Wnt Signaling.
Globus O, Evron T, Caspi M, Siman-Tov R, Rosin-Arbesfeld R
Scientific reports 2017 Dec 21;7(1):17995
Scientific reports 2017 Dec 21;7(1):17995
HTRA1 promoter variant differentiates polypoidal choroidal vasculopathy from exudative age-related macular degeneration.
Ng TK, Liang XY, Lai TY, Ma L, Tam PO, Wang JX, Chen LJ, Chen H, Pang CP
Scientific reports 2016 Jun 24;6:28639
Scientific reports 2016 Jun 24;6:28639
Protoporphyrins enhance oligomerization and enzymatic activity of HtrA1 serine protease.
Jo H, Patterson V, Stoessel S, Kuan CY, Hoh J
PloS one 2014;9(12):e115362
PloS one 2014;9(12):e115362
Angiographic features of transgenic mice with increased expression of human serine protease HTRA1 in retinal pigment epithelium.
Kumar S, Berriochoa Z, Ambati BK, Fu Y
Investigative ophthalmology & visual science 2014 May 22;55(6):3842-50
Investigative ophthalmology & visual science 2014 May 22;55(6):3842-50
Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1.
Jacobo SM, Deangelis MM, Kim IK, Kazlauskas A
Molecular and cellular biology 2013 May;33(10):1976-90
Molecular and cellular biology 2013 May;33(10):1976-90
Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways.
Wang N, Eckert KA, Zomorrodi AR, Xin P, Pan W, Shearer DA, Weisz J, Maranus CD, Clawson GA
PloS one 2012;7(6):e39446
PloS one 2012;7(6):e39446
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HTRA1/PRSS11 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB2916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA1/PRSS11 at approximately 51 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
