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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-29350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse brain, rat brain.
- Concentration
- 1.6 mg/mL
Submitted references Abelmoschus esculentus subfractions attenuate Aβ and tau by regulating DPP-4 and insulin resistance signals.
Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease.
Huang CN, Wang CJ, Lin CL, Li HH, Yen AT, Peng CH
BMC complementary medicine and therapies 2020 Dec 2;20(1):370
BMC complementary medicine and therapies 2020 Dec 2;20(1):370
Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease.
Hu X, Song C, Fang M, Li C
Experimental and therapeutic medicine 2018 Feb;15(2):1795-1802
Experimental and therapeutic medicine 2018 Feb;15(2):1795-1802
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of insulin degrading enzyme using 50 µg mouse liver lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of insulin degrading enzyme using 50 µg rat brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of insulin degrading enzyme using 30 µg of A) A431 (B) H1299 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDE was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IDE Polyclonal Antibody (Product # PA5-29350) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IDE Polyclonal Antibody (Product # PA5-29350). Various extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE Polyclonal Antibody (Product # PA5-29350) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of insulin degrading enzyme showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDE Polyclonal Antibody detects IDE protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IDE stained by IDE Polyclonal Antibody (Product # PA5-29350) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDE Polyclonal Antibody detects IDE protein at cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. IDE Polyclonal Antibody (Product # PA5-29350) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using insulin degrading enzyme (Product # PA5-29350) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 Effect of AE on attenuating p-Tau. SK-N-MC cells were incubated for 24 h with or without 50 muM of palmitate, and with different concentrations of F1 ( a ) and F2 ( b ). The expressions of IDE and p-Tau were analyzed by Western blotting. Data are presented as means +- SD ( n = 3) and analysed with ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the control. # p < 0.05, # p < 0.01, # p < 0.001 compared with the palmitate-treated only
