MAB12262

antibody from Abnova Corporation

Targeting: TIE1 JTK14, TIE

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB12262

Provider

Abnova Corporation

Product name

TIE1 monoclonal antibody, clone 8D12D2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant human TIE1.

Isotype

IgG

Antibody clone number

8D12D2

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [TIE1 (AA: 385-607)-hIgGFc transfected HEK293 cells] with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HepG2 cell lysate with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:500-1:2000 dilution.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ELISA analysis of TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:10000 dilution.

Validation comment

ELISA

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human ovarian cancer tissues (A) and kidney tissues (B) with TIE1 monoclonal antibody, clone 8D12D2 (Cat# MAB12262) at 1:200-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)