- PA5-27903 - Invitrogen Antibodies TIE1 antibody

Submitted references Mesenchymal Lineage Heterogeneity Underlies Nonredundant Functions of Pancreatic Cancer-Associated Fibroblasts.

Helms EJ, Berry MW, Chaw RC, DuFort CC, Sun D, Onate MK, Oon C, Bhattacharyya S, Sanford-Crane H, Horton W, Finan JM, Sattler A, Makar R, Dawson DW, Xia Z, Hingorani SR, Sherman MH
Cancer discovery 2022 Feb;12(2):484-501

LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis.

Xu M, Xu HH, Lin Y, Sun X, Wang LJ, Fang ZP, Su XH, Liang XJ, Hu Y, Liu ZM, Cheng Y, Wei Y, Li J, Li L, Liu HJ, Cheng Z, Tang N, Peng C, Li T, Liu T, Qiao L, Wu D, Ding YQ, Zhou WJ
Cell 2019 Sep 5;178(6):1478-1492.e20

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TIE1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human TIE1-transfected 293T cells. Samples were loaded onto a 5% SDS-PAGE gel and probed with a TIE1 polyclonal antibody (Product # PA5-27903) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TIE1 using 30 µg BCL-1 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TIE1 polyclonal antibody (Product # PA5-27903) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TIE1 in whole cell extracts (30 µg). Samples was separated by 5% SDS-PAGE and the membrane was probed with TIE1 Polyclonal antibody (Product # PA5-27903) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TIE1 Polyclonal Antibody detects TIE1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE1 Polyclonal Antibody (Product # PA5-27903) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-TIE1 Polyclonal Antibody (Product # PA5-27903) and a 130 kDa and 40 kDa band corresponding to Tyrosine-protein kinase receptor Tie-1 was observed in endothelial cell lines like HUVEC and HAEC but not in others. An uncharacterised band (*) at ~75 kDa was also detected across the cell lines. Whole cell extracts (40 µg lysate) of HAEC (Lane 1), HUVEC (Lane 2), HCT 116 (Lane 3), MDA-MB-231 (Lane 4) and Caco-2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1 (Product # PA5-27903) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-27903

Antibody data