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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MAB619 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Tie-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Tie-1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P35590- Isotype
- IgG
- Antibody clone number
- 88016
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Spatial and temporal distribution of Tie-1 and Tie-2 during very early development of the human placenta.
Kayisli UA, Cayli S, Seval Y, Tertemiz F, Huppertz B, Demir R
Placenta 2006 Jun-Jul;27(6-7):648-59
Placenta 2006 Jun-Jul;27(6-7):648-59
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tie-1 in HUVEC Human Cells. Tie-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tie-1 in Human Stomach. Tie-1 was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tie-1 in Human Gastric Cancer Tissue. Tie-1 was detected in immersion fixed paraffin-embedded sections of human gastric cancer tissue using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the pyloric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
