PA5-21528

antibody from Invitrogen Antibodies

Targeting: MRPS5 MRP-S5, S5mt

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21528

Provider

Invitrogen Antibodies

Product name

MRPS5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRPS5 using 30 µg of A) A431 (B) H1299 (C) HeLa S3 and D) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MRPS5 polyclonal antibody (Product # PA5-21528) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MRPS5 was performed by separating 50 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MRPS5 Polyclonal Antibody (Product # PA5-21528) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MRPS5 in methanol-fixed HeLa cells using a MRPS5 polyclonal antibody (Product # PA5-21528) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5 (Product # PA5-21528) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of MRPS5 was performed in paraffin-embedded mouse kidney tissue using MRPS5 Polyclonal Antibody (Product # PA5-21528) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MRPS5 Polyclonal Antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MRPS5 Polyclonal Antibody (Product # PA5-21528) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.