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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB6435 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse DGK-iota Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse DGK-iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha , -beta , -delta , -epsilon , -eta , -gamma , -kappa , -theta , or -zeta is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O75912- Isotype
- IgG
- Antibody clone number
- 648538
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse DGK-iota by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat DGK-iota Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
