PA5-69064

antibody from Invitrogen Antibodies

Targeting: SIDT2 CGI-40

Western blot

Antibody data

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Validation data

Product number: PA5-69064 - Provider product page
Provider: Invitrogen Antibodies
Product name: SIDT2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This target displays homology in the following species: Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 100%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

SIDT2 protein structure - PA5-69064 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Kidney damage and autophagolysosome accumulation in Sidt2 -/- mice. A Cre-LoxP system gene targeting schematic; B above is the exported sequence diagram of Sidt2 gene knockout mouse tail DNA; below is the sequencing map, the arrow indicating the location of the missing gene. Compared with WT mice, a 199 bp gene loss occurs in exon 2; C DNA level verification of Sidt2 (extracted from tail tissue). The primer-amplified product contains the base knockout region, shown as Sidt2 + /+ (WT), Sidt2 +/- , or Sidt2 -/- ; D protein level verification to detect Sidt2 protein expression levels by western blot; E kidney 24 h urine protein in WT and Sidt2 -/- mice; F ultra-micro-morphological structure of the kidneys of WT mice (a, f) and Sidt2 -/- mice (g-n). Compared with the WT mouse, the Sidt2 -/- mouse kidney displays foot process fusion, basement membrane thickening (g, h), renal tubular epithelial cell edema, microvilli damage (i, j), mitochondrial destruction (k, l), and autophagolysosome accumulation (m, n); G total number of renal autophagolysosomes in WT and Sidt2 -/- mice. * P < 0.05, ** P < 0.01.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Autophagy degradation disorder after Sidt2 deletion in mouse kidney cells. A LC3-II and P62 expression by MPC5 cells incubated for 16 h with 0, 10, 25, 50, 100, or 200 muM CQ shown by western blot; B LC3-II statistical chart of MPC5 cells; C P62 statistical chart of MPC5 cells; D SV40 MES 13 cell incubation for 16 h with 0, 10, 25, 50, 100, or 200 muM CQ. Western blot shows LC3-II and P62 expression; E LC3-II statistical chart of SV40 MES 13 cells; F SV40 MES 13 cell P62 statistical chart; G determination whether CQ induces the expression of key autophagy proteins before and after Sidt2 knockout in MPC5 cells; H statistical chart of ( G ); I changes in the autophagy flux in MPC5 cells on Sidt2 knockout (LC3-II + CQ/beta-Actin)/(LC3-II - CQ/beta-Actin) (ref. []); J determination whether chloroquine induces the expression of key autophagy proteins before and after Sidt2 knockout in SV40 MES 13 cells; K statistical chart of ( J ); L changes of the autophagy flux in SV40 MES 13 cells on Sidt2 knockout (LC3-II + CQ/beta-Actin)/(LC3-II - CQ/beta-Actin) (ref. []). * P < 0.05, ** P < 0.01, *** P < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Surface Plasmon Resonance of SIDT2 polyclonal antibody (Product # PA5-69064). Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 0.5, 5, and 50 µg/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333 nm peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.