14208-1-AP
antibody from Invitrogen Antibodies
Targeting: TRIM24
hTIF1, RNF82, TIF1, Tif1a
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 14208-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM24 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SKPAGADST HKVPVVMLEP IRIKQENSGP PENYDFPVVI VKQESDEESR PQNANYPRSI LTSLLLNSSQ SSTSEETVLR SDAPDSTGDQ PGLHQDNSSN GKSEWLDPSQ KSPLHVGETR KEDDPNEDWC AVCQNGGELL CCEKCPKVFH LSCHVPTLTN FPSGEWICTF CRDLSKPEVE YDCDAPSHNS EKKKTEGLVK LTPIDKRKCE RLLLFLYCHE MSLAFQDPVP LTVPDYYKII KNPMDLSTIK KRLQEDYSMY SKPEDFVADF RLIFQNCAEF NEPDSEVANA GIKLENYFEE LLKNLYPEKR FPKPEFRNES EDNKFSDDSD DDFVQPRKKR LKSIEERQLL K (701-1050 aa encoded by BC028689)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BGC-823 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 14208-1-AP (TRIM24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TRIM24 (IP:14208-1-AP, 4ug; Detection:14208-1-AP 1:800) with MCF-7 cells lysate 4000ug.