NB100-2596

antibody from Novus Biologicals

Targeting: TRIM24 hTIF1, RNF82, TIF1, Tif1a

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2596

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2596, RRID:AB_2209602

Product name

Rabbit Polyclonal TIF1 alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Dynamic regulation of Tgf-B signaling by Tif1γ: a computational approach.

Andrieux G, Fattet L, Le Borgne M, Rimokh R, Théret N
PloS one 2012;7(3):e33761

---

TIF1beta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner.

Seki Y, Kurisaki A, Watanabe-Susaki K, Nakajima Y, Nakanishi M, Arai Y, Shiota K, Sugino H, Asashima M
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 15;107(24):10926-31

---

Trim24 targets endogenous p53 for degradation.

Allton K, Jain AK, Herz HM, Tsai WW, Jung SY, Qin J, Bergmann A, Johnson RL, Barton MC
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 14;106(28):11612-6

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 Alpha/TRIM24 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TIF1 Alpha/TRIM24 antibody NB100-2596 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TIF1 alpha Antibody [NB100-2596] - Detection of Human and Mouse TIF1 alpha/TRIM24 on HeLa whole cell lysate using NB100-2596. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2597.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TIF1 alpha Antibody [NB100-2596] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TIF1 alpha/TRIM24 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - FFPE section of human breast tumor. Antibody used at a dilution of 1:250.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NB100-2596] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation Sequencing: TIF1 alpha Antibody [NB100-2596] - Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody A300-815A and analyzed by DNA sequencing. The figure illustrates the peak distribution of TIF1 Alpha/TRIM24 binding within a 250 Kb region of chromosome 19 as detected using anti-TIF1 Alpha/TRIM24 antibody A300-815A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.