Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- 500-P162-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLEC11A Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ AAAQARCTAR GGSLAQPADR QQMEALTRYL RAALAPYNWP VWLGVHDRRA EGLYLFENGQ RVSFFAWHRS PRPELGAQPS ASPHPLSPDQ PNGGTLENCV AQASDDGSWW DHDCQRRLYY VCEFPF.
- Concentration
- 0.1-1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human SCGF-alpha by Western Blot analysis CLEC11A Polyclonal Antibody (Product # 500-P162-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 n g/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human SCGF-alpha by Western Blot analysis CLEC11A Polyclonal Antibody (Product # 500-P162-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 n g/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human SCGF-alpha by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of CLEC11A Polyclonal Antibody (Product # 500-P162-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CLEC11A Polyclonal Antibody, Biotin (Product # 500-P162BT-1MG) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human SCGF-alpha.