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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
- Other assay [1]
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- Product number
- 11083-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFT57 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTAALAVVT TSGLEDGVPR SRGEGTGEVV LERGPGAAYH MFVVMEDLVE KLKLLRYEEE FLRKSNLKAP SRHYFALPTN PGEQFYMFCT LAAWLINKAG RPFEQPQEYD DPNATISNIL SELRSFGRTA DFPPSKLKSG YGEHVCYVLD CFAEEALKYI GFTWKRPIYP VEELEEESVA EDDAELTLNK VDEEFVEEET DNEENFIDLN VLKAQTYHLD MNETAKQEDI LESTTDAAEW SLEVERVLPQ LKVTIRTDNK DWRIHVDQMH QHRSGIESAL KETKGFLDKL HNEITRTLEK ISSREKYINN QLENLVQEYR AAQAQLSEAK ERYQQGNGGV TERTRLLSEV MEELEKVKQE MEEKGSSMTD GAPLVKIKQS LTKLKQETVE MDIRIGIVEH TLLQSKLKEK SNMTRNMHAT VIPEPATGFY (1-429 aa encoded by BC011899)
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse retina tissue were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of IFT57 antibody (11083-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT57 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MDCK cells using 11083-1-AP (IFT57 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IFT57 (IP:11083-1-AP, 5ug; Detection:11083-1-AP 1:800) with HEK-293 cells lysate 2000ug.
