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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [2]
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- Product number
- MAB80841 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal B7-H7/HHLA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human B7-H7/HHLA2 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of B7-H7/HHLA2 in HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and eGFP by Flow Cytometry. Detection of B7-H7/HHLA2 in HEK Human Cell Line Transfected with Human B7-H7/HHLA2 and eGFP by Flow Cytometry.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TMIGD2/CD28H Binding to B7-H7/ HHLA2-transfected HEK293 Human Cell Line is Blocked by Human B7-H7/HHLA2 Antibody. In a functional flow cytometry test, biotinylated recombinant human TMIGD2/ CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/ HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- B7-H7 Binding to TMIGD2/ CD28H Blocked by Human B7-H7/HHLA2 Antibody. In a Surface Plasmon Resonance test, Recombinant Human TMIGD2/ CD28H Fc Chimera (Catalog # 8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7, 5 μg/mL) (blue line). Binding is completely blocked (red) by 50 μg/mL of Mouse Anti-Human B7-H7/ HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 isotype (Catalog # MAB002) and irrelevant mouse monoclonal antibody were used as controls and did not affect TMIGD2/ CD28H binding to B7-H7/HHLA2 (not shown).
