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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF8084-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human B7-H7/HHLA2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human B7-H7/HHLA2 in direct ELISAs.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UM44- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human B7-H7/HHLA2 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidny cell line transfected with human B7-H7. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H7/HHLA2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- B7-H7/HHLA2 in Human Monocytes. B7-H7/HHLA2 was detected in immersion fixed human monocytes using Goat Anti-Human B7-H7/HHLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8084) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
