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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52042PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52042PU-N, RRID:AB_11147702
- Product name
- anti HHLA2 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human HHLA2
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HHLA2 Antibody (N-term) Cat.-No AP52042PU-N in MDA-MB453, CEM, HL-60, MDA-MB231 cell line lysates (35ug/lane). This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma reacted with AP52042PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB231 cells using AP52042PU-NÂ (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
