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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-92842 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OAT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VKGIQKYKAK IVFAAGNFWG RTLSAISSST DPTSYDGFGP FMPGFDIIPY NDLPALERAL QDPNVAAFMV EPIQGEAGVV VPDPGYLMGV RELCTRHQVL FIADEIQTGL ARTGRWLAVD YENVRPDIVL LGKALSGGLY PVSAVLCDDD IMLTIKPGEH GSTYGGNPLG CRVAIAALEV LEEENLAENA DKLGIILRNE LMKLPSDVVT AVRGKGLLNA IVIKETKDWD AWKVCLRLRD NGLLAKPTHG DIIRFAPPLV IKEDELRESI EIINKTILSF; Positive Samples: SW480, MCF7, 293T, A-549, HepG2, Mouse liver, Mouse lung, Mouse kidney, Rat kidney; Cellular Location: Mitochondrion matrix
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.
Key J, Torres-Odio S, Bach NC, Gispert S, Koepf G, Reichlmeir M, West AP, Prokisch H, Freisinger P, Newman WG, Shalev S, Sieber SA, Wittig I, Auburger G
Cells 2021 Nov 29;10(12)
Cells 2021 Nov 29;10(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OAT in extracts of various cell lines using OAT Polyclonal Antibody (Product # PA5-92842) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of OAT was performed in 200 µg extracts of MCF-7 cells using OAT Polyclonal Antibody (Product # PA5-92842). Western blot was performed from the immunoprecipitate using OAT Polyclonal Antibody.
