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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21838 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PRPSAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-21838 targets PRPP synthetase-associated protein 1 in IHC (P) and WB applications and shows reactivity with Human and mouse samples. The PA5-21838 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 356 of PRPSAP1 (Uniprot ID#Q14558). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRPP synthetase-associated protein 1 using 50 µg of mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PRPP synthetase-associated protein 1 polyclonal antibody (Product # PA5-21838) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRPP synthetase-associated protein 1 using 30µg of A) HeLa S3 and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PRPP synthetase-associated protein 1 polyclonal antibody (Product # PA5-21838) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PRPP synthetase-associated protein 1 in paraffin-embedded SW480 xenograft using a PRPP synthetase-associated protein 1 polyclonal antibody (Product # PA5-21838) at a dilution of 1:500.
