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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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- Product number
- GTX127928 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX127928, RRID:AB_2616069
- Product name
- hnRNP A2B1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
hnRNPs and ELAVL1 cooperate with uORFs to inhibit protein translation.
Resources for the Comprehensive Discovery of Functional RNA Elements.
Yu HI, Chou HC, Su YC, Lin LH, Lu CH, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Lee YR, Chan HL
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350
hnRNPs and ELAVL1 cooperate with uORFs to inhibit protein translation.
Zhang J, Kong L, Guo S, Bu M, Guo Q, Xiong Y, Zhu N, Qiu C, Yan X, Chen Q, Zhang H, Zhuang J, Wang Q, Zhang SS, Shen Y, Chen M
Nucleic acids research 2016 Oct 26;
Nucleic acids research 2016 Oct 26;
Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
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- Experimental details
- hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract E. 30 ?g A375 whole cell lysate/extract12% SDS-PAGEhnRNP A2B1 antibody (GTX127928) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extractC. 30 ?g C8D30 whole cell lysate/extractD. 30 ?g NIH-3T3 whole cell lysate/extractE. 30 ?g BCL-1 whole cell lysate/extractF. 30 ?g Raw264.7 whole cell lysate/extractG. 30 ?g C2C12 whole cell lysate/extract12% SDS-PAGEhnRNP A2B1 antibody (GTX127928) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract12% SDS-PAGEhnRNP A2B1 antibody (GTX127928) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000.
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX127928) diluted at 1:10000.
Supportive validation
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- Experimental details
- hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody (GTX127928) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 £gm.
Supportive validation
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- hnRNPA2B1 antibody immunoprecipitates hnRNPA2B1 protein in IP experiments. IP Sample: 4500 ?g A549 whole cell lysate/extract A. Immunoprecipitation of hnRNPA2B1 protein by 2 £gg of hnRNPA2B1 antibody (GTX127928) B. Control with 2 £gg of preimmune rabbit IgG The immunoprecipitated hnRNPA2B1 protein was detected by hnRNPA2B1 antibody diluted at 1:2000.
Supportive validation
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- hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. hnRNP A2B1 antibody (GTX127928) dilution: 1:500.
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- GeneTex (provider)
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- Experimental details
- hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. hnRNP A2B1 antibody (GTX127928) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. hnRNP A2B1 antibody (GTX127928) diluted at 1:500.
